CD107b / LAMP2 Monoklonaler Antikörper

CD107b / LAMP2 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

Unkonjugiert

CloneNo.

H4B4

Kat-Nr. : 65053-1-Ig

Synonyme

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

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Galerie der Validierungsdaten

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  IF Staining of HeLa using 65053-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65053-1-Ig (CD107b antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 65053-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107b / LAMP2 (65053-1-Ig, Clone:H4B4) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or Mouse IgG1 Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

65053-1-Ig bindet in IF, FC CD107b / LAMP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humane adhärente periphere Blutzellen
• Vollständiger Name: lysosomal-associated membrane protein 2
• Berechnetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC002965
• Gene symbol: LAMP2
• Gene ID (NCBI): 3920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).