CD107b / LAMP2 Monoklonaler Antikörper

CD107b / LAMP2 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2a

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

ABL-93

Kat-Nr. : 65052-1-Ig

Synonyme

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

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Galerie der Validierungsdaten

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  IF Staining of NIH/3T3 using 65052-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 65052-1-Ig (CD107b antibody) at dilution of 1:100 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

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  FC experiment of NIH/3T3 using 65052-1-Ig

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.50 ug Anti-Mouse CD107b (65052-1-Ig, Clone: ABL-93) (red) or 0.50 ug isotype control antibody (blue), and Fluorescein (FITC)-conjugated Affinipure Goat Anti-Rat IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

65052-1-Ig bindet in IF, FC CD107b / LAMP2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gereinigte Glykoproteine einer BALB/c-Mausembryo-3T3-Zelllinie
• Vollständiger Name: lysosomal-associated membrane protein 2
• GenBank-Zugangsnummer: BC138718
• Gene symbol: Lamp2
• Gene ID (NCBI): 16784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Borat-gepufferte Kochsalzlösung, pH 8,2.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Publikationen

Species	Application	Title
mouse	IF	Cell Rep Med; Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing; Authors - Dongming Lv