CD105 Monoklonaler Antikörper

CD105 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

Unkonjugiert

CloneNo.

MJ7/18

Kat-Nr. : 65048-1-Ig

Synonyme

AI528660, AI662476, CD105, endoglin, Eng, S endoglin

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Galerie der Validierungsdaten

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  IF Staining of mouse liver using 65048-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CD105 antibody (65048-1-Ig, Clone: MJ7/18 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of bEnd.3 using 65048-1-Ig

  1X10^6 bEnd.3 cells were surface stained with 0.25 ug Anti-Mouse CD105 (65048-1-Ig, Clone:MJ7/18) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 0.25 ug Control Antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Mauslebergewebe
• Erfolgreiche Detektion in FC: bEnd.3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

65048-1-Ig bindet in IF, FC CD105 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Entzündete Maushaut
• Vollständiger Name: endoglin
• GenBank-Zugangsnummer: BC029080
• Gene symbol: Eng
• Gene ID (NCBI): 13805
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Borat-gepufferte Kochsalzlösung, pH 8,2.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)