CD103 Monoklonaler Antikörper

CD103 Monoklonal Antikörper für FC

Wirt / Isotyp

hamster / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E7

Kat-Nr. : CL488-65047

Synonyme

A530055J10, alpha E1, alpha M290, aM290, CD103, Itgae

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using CL488-65047

  1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD103 (CL488-65047, Clone:2E7) or 0.5 ug Isotype Control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65047 bindet in FC CD103 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C57BL/6J Maus Intraepitheliale Lymphozyten (IEL)
• Vollständiger Name: integrin alpha E, epithelial-associated
• GenBank-Zugangsnummer: BC150690
• Gene symbol: Itgae
• Gene ID (NCBI): 16407
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD103, also known as integrin alpha-E (ITGAE) or integrin alpha-IEL, is a type I transmembrane integrin protein that binds integrin beta 7 to form Integrin alpha-E beta-7 which is a receptor for E-cadherin and mediates adhesion of T lymphocytes to epithelial cells (PMID: 7969453; 8468482). CD103 is expressed on intraepithelial lymphocyte (IEL) T cells (both alpha/beta T cells and gamma/delta T cells), some peripheral regulatory T cells (Tregs), lamina propria T cells, and a subset of dendritic cells in the gut mucosa and mesenteric lymph nodes (PMID: 12242333; 15608520; 16216890).