CD100 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CD100 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

1C1A6

Kat-Nr. : 66582-1-Ig

Synonyme

A8, BB18, C9orf164, CD100, coll 4, GR3, M sema G, SEMA4D, SEMAJ, Semaphorin 4D

---

Galerie der Validierungsdaten

• 

  WB analysis using 66582-1-Ig

  various cells were subjected to SDS PAGE followed by western blot with 66582-1-Ig (CD100 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66582-1-Ig

  WB result of CD100 antibody (66582-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD100 transfected HeLa cells.

• 

  IHC staining of human tonsillitis using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:450 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66582-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66582-1-Ig (CD100 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, K-562-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Appendizitis-Gewebe, humanes Lymphomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:225-1:900

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66582-1-Ig bindet in WB, IHC, ELISA CD100 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD100 fusion protein Ag26461
• Vollständiger Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC054500
• Gene symbol: SEMA4D
• Gene ID (NCBI): 10507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.

Publikationen

Species	Application	Title
human	WB	Oncol Lett; Low expression of SEMA4D as a potential predictive molecular marker of poor survival in patients with melanoma combined with liver cancer; Authors - Xiang Liu