CCT2 Monoklonaler Antikörper

CCT2 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H6C7

Kat-Nr. : CL488-68214

Synonyme

99D8.1, CCT beta, CCT2, CCTB, PRO1633, TCP 1 beta

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Galerie der Validierungsdaten

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  IF Staining of NIH/3T3 using CL488-68214

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 CCT2 antibody (CL488-68214, Clone: 2H6C7 ) at dilution of 1:200.

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  FC experiment of NIH/3T3 using CL488-68214

  1X10^6 NIH/3T3 cells were intracellularly stained with 1 ug CoraLite® Plus 488 Anti-Human CCT2 (CL488-68214, Clone:2H6C7) (red), or 1 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: NIH/3T3-Zellen
• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 1.00 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68214 bindet in IF, FC (Intra) CCT2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CCT2 fusion protein Ag19264
• Vollständiger Name: chaperonin containing TCP1, subunit 2 (beta)
• Berechnetes Molekulargewicht: 535 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50-58 kDa
• GenBank-Zugangsnummer: BC113516
• Gene symbol: CCT2
• Gene ID (NCBI): 10576
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with the molecular mass of 53 and 57 kDa. (PMID: 35699934)