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CCT2 Monoklonaler Antikörper

CCT2 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H6C7

Kat-Nr. : 68214-1-Ig

Synonyme

99D8.1, CCT beta, CCT2, CCTB, PRO1633, TCP 1 beta

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of various lysates using CCT2 Monoclonal antibody (68214-1-Ig)

    WB analysis using 68214-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68214-1-Ig (CCT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CCT2 Monoclonal antibody (68214-1-Ig)

    IF Staining of NIH/3T3 using 68214-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CCT2 antibody (68214-1-Ig, Clone: 2H6C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBLNCaP-Zellen, Hausschwein-Hirngewebe, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Kaninchenhirngewebe, Maushirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IFNIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

68214-1-Ig bindet in WB, IF, ELISA CCT2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Kaninchen, Maus, Ratte
In Publikationen genannte ReaktivitätMaus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen CCT2 fusion protein Ag19264
Vollständiger Name chaperonin containing TCP1, subunit 2 (beta)
Berechnetes Molekulargewicht 535 aa, 57 kDa
Beobachtetes Molekulargewicht 50-58 kDa
GenBank-ZugangsnummerBC113516
Gene symbol CCT2
Gene ID (NCBI) 10576
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)

Protokolle

Produktspezifische Protokolle
WB protocol for CCT2 antibody 68214-1-IgProtokoll herunterladen
IF protocol for CCT2 antibody 68214-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

Usher syndrome proteins ADGRV1 (USH2C) and CIB2 (USH1J) interact and share a common interactome containing TRiC/CCT-BBS chaperonins

Authors - Joshua Linnert
View Article