CCDC93 Polyklonaler Antikörper

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• KD/KO Validated

CCDC93 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 20861-1-AP

Synonyme

CCDC93

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Galerie der Validierungsdaten

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  WB analysis using 20861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20861-1-AP (CCDC93 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20861-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20861-1-AP (CCDC93 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 20861-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20861-1-AP (CCDC93 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 20861-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20861-1-AP (CCDC93 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 20861-1-AP

  IP Result of anti-CCDC93 (IP:20861-1-AP, 4ug; Detection:20861-1-AP 1:500) with L02 cells lysate 1800ug.

• 

  IHC staining of human colon using 20861-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20861-1-AP (CCDC93 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20861-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20861-1-AP (CCDC93 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Hodengewebe, Jurkat-Zellen, L02-Zellen, Maus-Kolongewebe
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

20861-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA CCDC93 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC93 fusion protein Ag14855
• Vollständiger Name: coiled-coil domain containing 93
• Berechnetes Molekulargewicht: 631 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC028609
• Gene symbol: CCDC93
• Gene ID (NCBI): 54520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.; Authors - Kerrie E McNally
mouse	WB	Nat Commun; CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.; Authors - Paulina Bartuzi
human	WB	J Cell Biol; Endosomal sorting of Notch receptors through COMMD9-dependent pathways modulates Notch signaling.; Authors - Haiying Li
human	WB,IF	Elife; Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes.; Authors - Sai Srinivas Panapakkam Giridharan
human	WB	Mol Metab; Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.; Authors - Liu Yang
human	CoIP	Mol Cancer Res; COMMD3-mediated endosomal trafficking of HER2 inhibits the progression of ovarian carcinoma; Authors - Shiqing Wang