CCDC50 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CCDC50 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 21082-1-AP

Synonyme

C3orf6, CCDC50, DFNA44, Protein Ymer, YMER

---

Galerie der Validierungsdaten

• 

  WB analysis using 21082-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21082-1-AP (CCDC50 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21082-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21082-1-AP (CCDC50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21082-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21082-1-AP (CCDC50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 21082-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21082-1-AP (CCDC50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21082-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21082-1-AP (CCDC50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21082-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21082-1-AP (CCDC50 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21082-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21082-1-AP (CCDC50 antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, L02-Zellen, Mauslungengewebe, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

21082-1-AP bindet in WB, IHC, IF, ELISA CCDC50 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC50 fusion protein Ag15293
• Vollständiger Name: coiled-coil domain containing 50
• Berechnetes Molekulargewicht: 482 aa, 56 kDa
• Beobachtetes Molekulargewicht: 36-38 kDa
• GenBank-Zugangsnummer: BC065004
• Gene symbol: CCDC50
• Gene ID (NCBI): 152137
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCDC50 encodes Ymer, an effector of epidermal growth factor (EGF)-mediated cell signaling that is ubiquitously expressed in different organs and has been suggested to inhibit down-regulation of the EGF receptor. CCDC50 act as a multifunctional regulator in NF-κB and Fas signaling pathways. In chronic lymphocytic leukaemia (CLL) and mantle cell lymphoma (MCL), CCDC50 is required for the survival of MCL and CLL cancer cells. Recently, CCDC50 was shown to promote hepatocellular carcinoma through the Ras/Foxo4 signaling pathway. (PMID: 34453126, PMID: 32612200, PMID: 17503326)

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IF	EMBO Rep; CCDC50 promotes tumor growth through regulation of lysosome homeostasis; Authors - Penghui Jia ; KD Validated