CEP83 Polyklonaler Antikörper

CEP83 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (2)

Kat-Nr. : 26013-1-AP

Synonyme

CCDC41, CEP83, NY REN 58

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 26013-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26013-1-AP (CEP83 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 26013-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26013-1-AP (CCDC41 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26013-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26013-1-AP (CCDC41 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of hTERT-RPE1 using 26013-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CEP83 antibody (26013-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

• 

  IF Staining of hTERT-RPE1 using 26013-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP83 antibody (26013-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 5 publications below

Produktinformation

26013-1-AP bindet in WB, IHC, IF, ELISA CEP83 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP83 fusion protein Ag22826
• Vollständiger Name: coiled-coil domain containing 41
• Berechnetes Molekulargewicht: 693 aa, 82 kDa
• Beobachtetes Molekulargewicht: 83 kDa, 68 kDa
• GenBank-Zugangsnummer: BC125087
• Gene symbol: CCDC41
• Gene ID (NCBI): 51134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP83 (centrosomal protein, 83 kDa), also known as CCDC41 (coiled-coil domain containing 41), is required for ciliary vesicle docking to the mother centriole. It is a key component of the centriolar distal appendages. CCDC41 may collaborate with IFT20 in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium during the initiation of primary cilium assembly (PMID: 23530209). Alternative splicing results in transcript variants encoding two isoforms with calculated molecular weights of 83 kDa and 68 kDa, respectively.

Publikationen

Species	Application	Title
mouse	WB,IF	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
human	WB,IF	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	IF	PLoS Biol; The evolutionary conserved proteins CEP90, FOPNL, and OFD1 recruit centriolar distal appendage proteins to initiate their assembly; Authors - Pierrick Le Borgne
human	IF	Cell Mol Life Sci; Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.; Authors - Delowar Hossain
human	WB	J Biol Chem; The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.; Authors - Adrian Cuenca
human	WB	bioRxiv; A hierarchical pathway for assembly of the distal appendages that organize primary cilia; Authors - Tomoharu Kanie

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (03-01-2022)

HEK293 cells stained for Hoechst (DNA marker, in green), Cep83 (mother centriole distal appendage marker, in magenta) and g-Tubulin (pericentriolar matrix marker, in green). HEK293 cells were plated on Poly-lysine coated coverslips and fixed in cold methanol for 2' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HEK293 cells (Human embryonic kidney 293 cells)

CEP83 Antibody Immunofluorescence validation (1:200 dilution) in HEK293 cells (Human embryonic kidney 293 cells) (Cat no:26013-1-AP)

FH

Sarah (Verified Customer) (07-03-2019)

Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-CCDC41 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U251/Jurkat

CEP83 Antibody Western Blot, validation (1:1000 dilution) in U251/Jurkat (Cat no:26013-1-AP)