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CCDC28A Polyklonaler Antikörper

CCDC28A Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14807-1-AP

Synonyme

C6orf80, CCDC28A, CCRL1AP

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using CCDC28A Polyclonal antibody (14807-1-AP)

    WB analysis using 14807-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CCDC28A Polyclonal antibody (14807-1-AP)

    WB analysis of HeLa using 14807-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CCDC28A Polyclonal antibody (14807-1-AP)

    WB analysis of mouse thymus using 14807-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14807-1-AP (CCDC28A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CCDC28A Polyclonal antibody (14807-1-AP)

    IF Staining of MCF-7 using 14807-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CCDC28A antibody (14807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CCDC28A Polyclonal antibody (14807-1-AP)

    IF Staining of MCF-7 using 14807-1-AP

    Immunofluorescent analysis of MCF-7 cells, using CCDC28A antibody 14807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBA375-Zellen, HeLa-Zellen, Jurkat-Zellen, Maus-Thymusgewebe, MCF-7-Zellen
Erfolgreiche Detektion in IFMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunfluoreszenz (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14807-1-AP bindet in WB, IF, ELISA CCDC28A und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CCDC28A fusion protein Ag6529
Vollständiger Name coiled-coil domain containing 28A
Berechnetes Molekulargewicht 30 kDa
Beobachtetes Molekulargewicht70 kDa
GenBank-ZugangsnummerBC000758
Gene symbol CCDC28A
Gene ID (NCBI) 25901
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
WB protocol for CCDC28A antibody 14807-1-APProtokoll herunterladen
IF protocol for CCDC28A antibody 14807-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen