CCDC171 Polyklonaler Antikörper

CCDC171 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 28433-1-AP

Synonyme

bA536D16.1, bA778P13.1, C9orf93, CCDC171, DKFZp686P12113, FLJ39267, FLJ46740

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Galerie der Validierungsdaten

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  WB analysis using 28433-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28433-1-AP (CCDC171 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28433-1-AP bindet in WB, ELISA CCDC171 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC171 fusion protein Ag28926
• Vollständiger Name: chromosome 9 open reading frame 93
• Berechnetes Molekulargewicht: 153 kDa
• Beobachtetes Molekulargewicht: 142 kDa
• GenBank-Zugangsnummer: NM_173550
• Gene symbol: C9orf93
• Gene ID (NCBI): 203238
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (08-03-2022)

U2OS cells were lysates with RIPA buffer. 200ng for condition was loaded on the gel. . Proteins were run on a 12-4% gradient polyacrylamide gel at 180V constant. Gel was transferred on nitrocellulose filter at 300mA constant. Blots were then blocked in 5%milk in TBS-0,1%Tween for 30 min and then incubated with CCDC171 primary antibody (1:1000 dilution). Anti-rabbit -HRP conjugated antibody (1:2000) was used to detect CCDC171 signal (140-5kDa). b-tubulin (55kDa) was used as a loading control. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: U2OS

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS (Human Osteosarcoma) cells

CCDC171 Antibody Western Blot validation (1:1000 dilution) in U2OS (Human Osteosarcoma) cells (Cat no:28433-1-AP)