CCDC124 Polyklonaler Antikörper

CCDC124 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27106-1-AP

Synonyme

CCDC124

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 27106-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 27106-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 27106-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27106-1-AP (CCDC124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 27106-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 27106-1-AP (CCDC124 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 27106-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 27106-1-AP (CCDC124 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Plazenta-Gewebe, Mauslebergewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27106-1-AP bindet in WB, IF,ELISA CCDC124 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCDC124 fusion protein Ag25930
• Vollständiger Name: coiled-coil domain containing 124
• Beobachtetes Molekulargewicht: 30-32 kDa
• GenBank-Zugangsnummer: BC013949
• Gene symbol: CCDC124
• Gene ID (NCBI): 115098
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCDC124 is a novel centrosomal and midbody protein that is highly conserved among species. It is ubiquitously expressed with high expression in brain, placenta, liver, spleen, and prostate. At telophase, CCDC124 dissociates from centrosomes and relocalizes to the midzone, subsequently accumulating at the midbody at cytokinesis. It interacts with the Ras-guanine nucleotide exchange factor 1B (RasGEF1B) and is involved in cytokinesis.