CEP89, CCDC123 Monoklonaler Antikörper

CEP89, CCDC123 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F12C5

Kat-Nr. : 68112-1-Ig

Synonyme

CCDC123, FLJ14640

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Galerie der Validierungsdaten

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  WB analysis of Neuro-2a using 68112-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68112-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CEP89, CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

• 

  WB analysis using 68112-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68112-1-Ig (CCDC123 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse eye using 68112-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse eye using 68112-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CEP89, CCDC123 antibody (68112-1-Ig, Clone: 1F12C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, A549-Zellen, Neuro-2a-Zellen, SH-SY5Y-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IF: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68112-1-Ig bindet in WB, IF, ELISA CEP89, CCDC123 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CEP89, CCDC123 fusion protein Ag28339
• Vollständiger Name: coiled-coil domain containing 123
• Berechnetes Molekulargewicht: 783 aa, 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC136328
• Gene symbol: CCDC123
• Gene ID (NCBI): 84902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCDC123 (as known as CEP123), also named as CEP89, encodes for a protein required for ciliogenesis. It plays a role in mitochondrial metabolism by modulating complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with CEP290 (PMID:23575228, 23789104, 23348840).