CCAR1 Polyklonaler Antikörper

CCAR1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 25232-1-AP

Synonyme

RP11 437A18.1, DIS, Death inducer with SAP domain, CARP-1, CARP1

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  WB analysis using 25232-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25232-1-AP (CCAR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 25232-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25232-1-AP (CCAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 25232-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (25232-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of A431 using 25232-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCAR1 antibody (25232-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Mammakarzinomgewebe, Maus-Kolongewebe, Ratten-Kolongewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

25232-1-AP bindet in WB, IHC, IF/ICC, ELISA CCAR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCAR1 fusion protein Ag18970
• Vollständiger Name: cell division cycle and apoptosis regulator 1
• Berechnetes Molekulargewicht: 1150 aa, 133 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC132725
• Gene symbol: CCAR1
• Gene ID (NCBI): 55749
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cell cycle and apoptosis regulator 1 (CCAR1) was originally identified from a functional screening and implicated in retinoid-induced cell cycle arrest and apoptosis. It has been shown that, in breast cancer cells, CCAR1 regulates apoptosis induced by various agents, including chemotherapeutics adriamycin, etoposide, and EGFR inhibitor. It is also involved in mediating anchorage-independent growth of colon carcinoma cells. In addition, CCAR1 plays the role as a co-activator of nuclear receptors to recruit mediator complex. It was found to associate with components of the Mediator and p160 coactivator complexes and is recruited to endogenous NR target genes. CCAR1 is also a binding partner of b-catenin and assists b-catenin in transcriptional activation of Wnt target genes.

Publikationen

Species	Application	Title
human	WB	Cancer Med; circTUBD1-hnRNPK Regulates the Proliferation and Migration of LSCC by Targeting CCAR1; Authors - Yufeng Xu