CC2D1B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CC2D1B Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 20774-1-AP

Synonyme

CC2D1B, Freud-2, KIAA1836, LGD1

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 20774-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20774-1-AP (CC2D1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20774-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20774-1-AP (CC2D1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20774-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20774-1-AP (CC2D1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 20774-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20774-1-AP (CC2D1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20774-1-AP

  IP Result of anti-CC2D1B (IP:20774-1-AP, 4ug; Detection:20774-1-AP 1:500) with mouse brain tissue lysate 2560ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Plazenta-Gewebe, Mausnierengewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

20774-1-AP bindet in WB, IP, IF, ELISA CC2D1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CC2D1B fusion protein Ag14767
• Vollständiger Name: coiled-coil and C2 domain containing 1B
• Berechnetes Molekulargewicht: 858 aa, 94 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC007912
• Gene symbol: CC2D1B
• Gene ID (NCBI): 200014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CC2D1B/Freud2 and CC2D1A/Freud1 are two homologs belonging to the evolutionary conserved Lgd protein family that is involved in endosomal trafficking. CC2D1B has been identified as the novel repressor of postsynaptic 5-HT1A expression. The predicted molecular weight of CC2D1B is 89 kDa, but the observed molecular weight is around 120 kDa, which may be caused by post-translational modification. This antibody is specific to CC2D1B, which has been confirmed by siRNA test. (19423080 , 2672064)

Publikationen

Species	Application	Title
mouse	WB,IF	PLoS Genet; The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling.; Authors - Nadja Drusenheimer ; KO Validated
mouse	WB	Front Mol Neurosci; Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System.; Authors - Jenica Acheta ; KO Validated
rat	WB,IF	Front Mol Neurosci; YAP and TAZ Regulate Cc2d1b and Purβ in Schwann Cells.; Authors - Belin Sophie ; KD Validated
human	IF	Biol Pharm Bull; Icariin regulates EMT and stem cell-like character in breast cancer through modulating lncRNA NEAT1/TGFβ/SMAD2 signaling pathway; Authors - Bo Song

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Abigail (Verified Customer) (09-16-2019)

It worked well for us

• Applications: Western Blot, Immunoprecipitation,
• Cell Tissue Type: Murine Hippocampus and cortex