Calpastatin Monoklonaler Antikörper

Calpastatin Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1G12A2

Kat-Nr. : 67107-1-Ig

Synonyme

BS 17, Calpain inhibitor, calpastatin, CAST, Sperm BS 17 component

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 67107-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (CAST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67107-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67107-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67107-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67107-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67107-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (Calpastatin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 67107-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpastatin antibody (67107-1-Ig, Clone: 1G12A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 67107-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Calpastatin (67107-1-Ig, Clone:1G12A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, LNCaP-Zellen, MCF-7-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

67107-1-Ig bindet in WB, IF, FC, ELISA Calpastatin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Calpastatin fusion protein Ag28669
• Vollständiger Name: calpastatin
• Berechnetes Molekulargewicht: 667 aa, 72 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC013579
• Gene symbol: CAST
• Gene ID (NCBI): 831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis.; Authors - Fengming Shen
mouse	WB	Pharmacol Res; Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.; Authors - Juan Wang