Calsequestrin 2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Calsequestrin 2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 18422-1-AP

Synonyme

Calsequestrin 2, CASQ2, PDIB2

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Galerie der Validierungsdaten

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  WB analysis of human heart using 18422-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18422-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 18422-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18422-1-AP (Calsequestrin 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18422-1-AP (Calsequestrin 2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18422-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of C2C12 using 18422-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed C2C12 cell using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Hausschwein-Herzgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: C2C12 cell

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

18422-1-AP bindet in WB, IHC, IF, ELISA Calsequestrin 2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calsequestrin 2 fusion protein Ag13246
• Vollständiger Name: calsequestrin 2 (cardiac muscle)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC022288
• Gene symbol: CASQ2
• Gene ID (NCBI): 845
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

Publikationen

Species	Application	Title
mouse	WB	EMBO Mol Med; SK4 K(+) channels are therapeutic targets for the treatment of cardiac arrhythmias.; Authors - Shiraz Haron-Khun
mouse	WB,IF	JCI Insight; The Purkinje-myocardial junction is the anatomical origin of ventricular arrhythmia in CPVT.; Authors - Daniel J Blackwell ; KO Validated
mouse	WB,IF	Heart Rhythm; Viral delivered gene therapy to treat catecholaminergic polymorphic ventricular tachycardia (CPVT2) in mouse models.; Authors - Efrat Kurtzwald-Josefson ; KO Validated
human	WB	Stem Cell Reports; A Human Stem Cell Model of Fabry Disease Implicates LIMP-2 Accumulation in Cardiomyocyte Pathology.; Authors - Matthew J Birket
		Mol Biol Cell; Fer1l6 Is Essential for the Development of Vertebrate Muscle Tissue in Zebrafish.; Authors - Josephine A Bonventre
mouse	WB	JCI Insight; Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy.; Authors - Yingchao Shi