Caspase 9/p35/p10 Polyklonaler Antikörper

Caspase 9/p35/p10 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 23821-1-AP

Synonyme

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 23821-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of UV treated Hela using 23821-1-AP

  UV treated Hela cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of UV treated Hela using 23821-1-AP

  UV treated Hela cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23821-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23821-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 23821-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 23821-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 23821-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 23821-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

• 

  IF Staining of HUVEC using 23821-1-AP

  Immunofluorescent analysis of HUVEC cells using 23821-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen,
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

23821-1-AP bindet in WB, IHC, IF,ELISA Caspase 9/p35/p10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Caspase 9/p35/p10 fusion protein Ag20813
• Vollständiger Name: caspase 9, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC002452
• Gene symbol: CASP9
• Gene ID (NCBI): 842
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. CASP9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade

Publikationen

Species	Application	Title
mouse	WB	J Cell Physiol; Alpha-Lipoic Acid Promotes Osteoblastic Formation in H2 O2 -Treated MC3T3-E1 Cells and Prevents Bone Loss in Ovariectomized Rats.; Authors - Chao Fu