CASK Polyklonaler Antikörper

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• KD/KO Validated

CASK Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55277-1-AP

Synonyme

CAGH39, CASK, CMG, FGS4, hCASK, LIN2, MICPCH, Protein lin 2 homolog, TNRC8

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Galerie der Validierungsdaten

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  WB analysis using 55277-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 55277-1-AP

  WB result of CASK antibody (55277-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CASK transfected A549 cells.

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  WB analysis of mouse liver using 55277-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55277-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 55277-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 55277-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, Mauslebergewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55277-1-AP bindet in WB, IHC, ELISA CASK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: calcium/calmodulin-dependent serine protein kinase (MAGUK family)
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa, 50-55 kDa
• GenBank-Zugangsnummer: NM_003688
• Gene symbol: CASK
• Gene ID (NCBI): 8573
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CASK, also named as LIN2, belongs to the MAGUK family. It is a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. It contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. CASK binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. It may mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) . Defects in CASK are the cause of FG syndrome type 4 (FGS4). This antibody can recognize all the isoform of CASK.