CARD11 Monoklonaler Antikörper

CARD11 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

3F5H6

Kat-Nr. : 68269-1-Ig

Synonyme

BIMP3, CARD11, Carma 1, CARMA1

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Galerie der Validierungsdaten

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  WB analysis using 68269-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68269-1-Ig (CARD11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 68269-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 68269-1-Ig (CARD11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of L02 using 68269-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using CARD11 antibody (68269-1-Ig, Clone: 3F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MOLT-4-Zellen, Jurkat-Zellen, K-562-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68269-1-Ig bindet in WB, IF, ELISA CARD11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CARD11 fusion protein Ag16468
• Vollständiger Name: caspase recruitment domain family, member 11
• Berechnetes Molekulargewicht: 1154 aa, 133 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC111719
• Gene symbol: CARD11
• Gene ID (NCBI): 84433
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.