Calcyphosine Polyklonaler Antikörper

Calcyphosine Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16926-1-AP

Synonyme

Calcyphosin, calcyphosine, CAPS, CAPS1

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Galerie der Validierungsdaten

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  WB analysis of human spleen using 16926-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 16926-1-AP (Calcyphosine antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 16926-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 16926-1-AP (Calcyphosine antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16926-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 16926-1-AP (Calcyphosine antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 16926-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16926-1-AP (Calcyphosine antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 16926-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16926-1-AP (Calcyphosine antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, humanes Kolongewebe, humanes Milzgewebe
• Erfolgreiche Detektion in IHC: humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16926-1-AP bindet in WB, IHC, ELISA Calcyphosine und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calcyphosine fusion protein Ag10330
• Vollständiger Name: calcyphosine
• Berechnetes Molekulargewicht: 189 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC080599
• Gene symbol: CAPS
• Gene ID (NCBI): 828
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

calcyphosine is a calcium-binding protein, which may play a role in the regulation of ion transport. Calcyphosine (CAPS) was initially identified in the canine thyroid but has also been detected in humans and other mammals. CAPS synthesis is increased by thyroid‐stimulating hormone and cAMP analogs that promote cell proliferation and maintain cell differentiation. Recently, CAPS was found to be involved in many kinds of tumor.