CAPRIN1 Monoklonaler Antikörper

CAPRIN1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

6B10F11

Kat-Nr. : CL594-66352

Synonyme

caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI

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Galerie der Validierungsdaten

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  IF Staining of HeLa using CL594-66352

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated CAPRIN1 antibody (CL594-66352, Clone: 6B10F11 ) at dilution of 1:100.

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  IF Staining of HeLa using CL594-66352

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CAPRIN1 antibody (CL594-66352, Clone: 6B10F11 ) at dilution of 1:800.

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  IF Staining of HeLa using CL594-66352

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66352 (CAPRIN1 antibody) at dilution of 1:100.

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  FC experiment of NIH/3T3 using CL594-66352

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CAPRIN1 (CL594-66352, Clone:6B10F11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66352 bindet in IF, FC (Intra) CAPRIN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CAPRIN1 fusion protein Ag7413
• Vollständiger Name: cell cycle associated protein 1
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: BC001731
• Gene symbol: CAPRIN1
• Gene ID (NCBI): 4076
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C