CAPNS1 Monoklonaler Antikörper

CAPNS1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B6G6

Kat-Nr. : CL488-67838

Synonyme

30K, Calpain regulatory subunit, Calpain small subunit 1, calpain, small subunit 1, CALPAIN4, CANP, CANP small subunit, CANPS, CAPN4, CAPNS, CAPNS1, CDPS, CSS1

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Galerie der Validierungsdaten

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  IF Staining of A431 using CL488-67838

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 CAPNS1 antibody (CL488-67838, Clone: 2B6G6 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-67838

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CAPNS1 (CL488-67838, Clone:2B6G6) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: A431-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67838 bindet in IF, FC (Intra) CAPNS1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CAPNS1 fusion protein Ag21885
• Vollständiger Name: calpain, small subunit 1
• Berechnetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC011903
• Gene symbol: CAPNS1
• Gene ID (NCBI): 826
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAPNS1 is also named as CANP, CDPS, CSS1, CANPS, CAPN4 and CALPAIN4. It is a member of the calpain small subunit family, and belongs to the calcium-dependent family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. The small regulatory subunit consists of an N-terminal domain, containing about 30% glycine residues and a C-terminal Ca-binding domain (PMID: 9228946). This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C