CAPNS1 Monoklonaler Antikörper

CAPNS1 Monoklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

CloneNo.

2B6G6

Kat-Nr. : 67838-1-Ig

Synonyme

30K, Calpain regulatory subunit, Calpain small subunit 1, calpain, small subunit 1, CALPAIN4, CANP, CANP small subunit, CANPS, CAPN4, CAPNS, CAPNS1, CDPS, CSS1

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Galerie der Validierungsdaten

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  WB analysis of A375 using 67838-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67838-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67838-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67838-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67838-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67838-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 67838-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 67838-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CAPNS1 antibody (67838-1-Ig, Clone: 2B6G6 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A431-Zellen, HeLa-Zellen, K-562-Zellen, PC-12-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67838-1-Ig bindet in WB, IF,ELISA CAPNS1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CAPNS1 fusion protein Ag21885
• Vollständiger Name: calpain, small subunit 1
• Berechnetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC011903
• Gene symbol: CAPNS1
• Gene ID (NCBI): 826
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.