CAP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CAP2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 15865-1-AP

Synonyme

Adenylyl cyclase associated protein 2, CAP 2, CAP2

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Galerie der Validierungsdaten

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  WB analysis of human kidney using 15865-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15865-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 15865-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15865-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15865-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15865-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15865-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15865-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15865-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15865-1-AP (CAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 15865-1-AP

  IP Result of anti-CAP2 (IP:15865-1-AP, 4ug; Detection:15865-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 15865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15865-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 15865-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15865-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15865-1-AP (CAP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of U2OS using 15865-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 15865-1-AP (CAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U2OS using 15865-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CAP2 antibody (15865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HepG2-Zellen, humanes Nierengewebe, Mausherzgewebe, Maushodengewebe, Mausnierengewebe, Maus-Skelettmuskelgewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15865-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA CAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAP2 fusion protein Ag8477
• Vollständiger Name: CAP, adenylate cyclase-associated protein, 2 (yeast)
• Berechnetes Molekulargewicht: 477 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC008481
• Gene symbol: CAP2
• Gene ID (NCBI): 10486
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAPs (CAP1 and CAP2) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is predominantly expressed in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Overexpression of CAP2 has been reported in many cancers, including hepatocellular carcinoma (HCC), human breast cancer, and malignant melanoma.

Publikationen

Species	Application	Title
human	WB,IHC,IF,CoIP	J Clin Invest; CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages; Authors - Guohao Zhang ; KD Validated
human	WB	Int J Mol Sci; Analysis of mRNA and Protein Levels of CAP2, DLG1 and ADAM10 Genes in Post-Mortem Brain of Schizophrenia, Parkinson's and Alzheimer's Disease Patients.; Authors - Anna Di Maio
mouse,rat	WB,IF	Commun Biol; CAP2 is a regulator of actin pointed end dynamics and myofibrillogenesis in cardiac muscle.; Authors - Mert Colpan ; KO Validated
mouse	WB	Sci Rep; Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblasts.; Authors - Lara-Jane Kepser
		Oncol Rep; Expression status of cyclase‑associated protein 2 as a prognostic marker for human breast cancer.; Authors - Lihua Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anil K (Verified Customer) (06-10-2022)

Saw a single band in the mice brain lysates

• Applications: Western Blot
• Cell Tissue Type: brain