CAMSAP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CAMSAP2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

39

Kat-Nr. : 17880-1-AP

Synonyme

CAMSAP1L1, CAMSAP2, KIAA1078

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Galerie der Validierungsdaten

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  WB analysis using 17880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17880-1-AP (CAMSAP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17880-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17880-1-AP (CAMSAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 17880-1-AP

  IP Result of anti-CAMSAP2 (IP:17880-1-AP, 4ug; Detection:17880-1-AP 1:3000) with PC-3 cells lysate 5000ug.

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  IHC staining of human kidney using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse testis using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 17880-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17880-1-AP (CAMSAP2 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, Maushirngewebe, PC-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Plazenta-Gewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 20 publications below
• IF: See 29 publications below

Produktinformation

17880-1-AP bindet in WB, IP, IHC, IF, ELISA CAMSAP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAMSAP2 fusion protein Ag11794
• Vollständiger Name: calmodulin regulated spectrin-associated protein 1-like 1
• Berechnetes Molekulargewicht: 1478 aa, 167 kDa
• Beobachtetes Molekulargewicht: 168 kDa
• GenBank-Zugangsnummer: BC065508
• Gene symbol: CAMSAP1L1
• Gene ID (NCBI): 23271
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAMSAP2, also known as CAMSAP1L1, is a microtubule minus-end binding protein, and is required for proper microtubule organization in neurons. CAMSAP2 stabilizes noncentrosomal microtubules and is important for neuronal polarity, axon specification, and dendritic branch formation.

Publikationen

Species	Application	Title
human	IF	Nat Struct Mol Biol; A chemical genetics approach to examine the functions of AAA proteins.; Authors - Tommaso Cupido
rat	WB,IF	Neuron; Microtubule Minus-End Binding Protein CAMSAP2 Controls Axon Specification and Dendrite Development.; Authors - Kah Wai Yau ; KD Validated
mouse	IF	Neuron; Katanin p80 regulates human cortical development by limiting centriole and cilia number.; Authors - Wen F Hu
rat	IF	Neuron; TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays.; Authors - Sam F B van Beuningen
human	WB	Neuron; Memo1-Mediated Tiling of Radial Glial Cells Facilitates Cerebral Cortical Development.; Authors - Naoki Nakagawa
mouse	IF	Neuron; Dynein Regulator NDEL1 Controls Polarized Cargo Transport at the Axon Initial Segment.; Authors - Marijn Kuijpers