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  • Featured Product
  • KD/KO Validated

CALD1 Monoklonaler Antikörper

CALD1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

1E1C11

Kat-Nr. : CL555-66693

Synonyme

CAD, CALD1, Caldesmon, caldesmon 1, CDM, H CAD, L CAD, NAG22

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated CALD1 Monoclonal antibody (CL555-66693)

    IF Staining of HeLa using CL555-66693

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 CALD1 antibody (CL555-66693, Clone: 1E1C11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite®555-conjugated CALD1 Monoclonal antibody (CL555-66693)

    FC experiment of NIH/3T3 using CL555-66693

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human CALD1 (CL555-66693, Clone:1E1C11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCNIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-66693 bindet in IF, FC (Intra) CALD1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen CALD1 fusion protein Ag13676
Vollständiger Name caldesmon 1
Berechnetes Molekulargewicht 538 aa, 63 kDa
Beobachtetes Molekulargewicht 70 kDa~75 kDa
GenBank-ZugangsnummerBC040354
Gene symbol CALD1
Gene ID (NCBI) 800
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
IF protocol for CL555 CALD1 antibody CL555-66693Protokoll herunterladen
FC protocol for CL555 CALD1 antibody CL555-66693Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen