CAD Polyklonaler Antikörper

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• KD/KO Validated

CAD Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16617-1-AP

Synonyme

CAD

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 16617-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16617-1-AP (CAD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 16617-1-AP

  IP Result of anti-CAD (IP:16617-1-AP, 4ug; Detection:16617-1-AP 1:500) with HEK-293 cells lysate 2800ug.

• 

  IHC staining of mouse liver using 16617-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16617-1-AP (CAD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16617-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16617-1-AP (CAD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 16617-1-AP

  Immunofluorescent analysis of HEK-293 cells using 16617-1-AP (CAD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

16617-1-AP bindet in WB, IP, IHC, IF, ELISA CAD und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAD fusion protein Ag9912
• Vollständiger Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
• Berechnetes Molekulargewicht: 2225 aa, 243 kDa
• Beobachtetes Molekulargewicht: 240 kDa
• GenBank-Zugangsnummer: BC014178
• Gene symbol: CAD
• Gene ID (NCBI): 790
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides (PMID:25422319). CAD initiates and controls the flflux through the pathway and is regulated by allosteric effectors and phosphorylations through different signaling cascades (PMID:12438317). Moreover, CAD is found upregulated in tumors, while mutations that compromise its function are the cause of a severe infantile epileptic disease (PMID:36677714).

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
human	WB	Theranostics; Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription.; Authors - Gengyi Zou
human	WB, IHC	PLoS Biol; Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.; Authors - Yajing Lv
human	WB,IHC	Front Cell Dev Biol; Identification of Key Genes Related With Aspartic Acid Metabolism and Corresponding Protein Expression in Human Colon Cancer With Postoperative Prognosis and the Underlying Molecular Pathways Prediction.; Authors - Weixuan Sun
human	WB	Carcinogenesis; Synergistic Targeting of CHK1 and mTOR in MYC-driven tumors.; Authors - Xiaoxue Song ; KD Validated
human	WB	Cancer Sci; Apelin-mediated deamidation of HMGA1 promotes tumorigenesis by enhancing SREBP1 activity and lipid synthesis; Authors - Yihan Zhu