CA12 Polyklonaler Antikörper

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• KD/KO Validated

CA12 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 15180-1-AP

Synonyme

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, HsT18816, Tumor antigen HOM RCC 3.1.3

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Galerie der Validierungsdaten

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  WB analysis using 15180-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15180-1-AP

  WB result of CA12 antibody (15180-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA12 transfected MCF-7 cells.

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  WB analysis of A549 using 15180-1-AP

  WB result of CA12 antibody (15180-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA12 transfected A549 cells.

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  WB analysis of BxPC-3 using 15180-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15180-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15180-1-AP (CA12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15180-1-AP (CA12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15180-1-AP (CA12 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15180-1-AP (CA12 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HEK-293 using 15180-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug CA12 antibody (15180-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, BxPC-3-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

15180-1-AP bindet in WB, IP, IHC, IF, FC, ELISA CA12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CA12 fusion protein Ag7107
• Vollständiger Name: carbonic anhydrase XII
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 44 kDa, 75-80 kDa
• GenBank-Zugangsnummer: BC000278
• Gene symbol: CA12
• Gene ID (NCBI): 771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

Publikationen

Species	Application	Title
human	WB, IF	J Exp Med; Large pH oscillations promote host defense against human airways infection.; Authors - Dusik Kim
human	WB	Mol Oncol; Mechanism of irradiation-induced mammary cancer metastasis: A role for SAP-dependent Mkl1 signaling.; Authors - Maria B Asparuhova
human	WB,IF	Hum Mol Genet; Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease.; Authors - Melissa Lee
human	IHC	Int J Mol Sci; Perfusion-Based Bioreactor Culture and Isothermal Microcalorimetry for Preclinical Drug Testing with the Carbonic Anhydrase Inhibitor SLC-0111 in Patient-Derived Neuroblastoma.; Authors - Zihe Huo
human	WB,IF	J Enzyme Inhib Med Chem; Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5.; Authors - Soyoung Hwang
human	WB	Front Oncol; Integrative Proteo-Genomic Analysis for Recurrent Survival Prognosis in Colon Adenocarcinoma.; Authors - FeiYan Ai