C7orf47 Polyklonaler Antikörper

C7orf47 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 24214-1-AP

Synonyme

C7orf47, UPF0683 protein C7orf47

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Galerie der Validierungsdaten

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  IHC staining of human breast cancer using 24214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 24214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 24214-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 24214-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

24214-1-AP bindet in IHC, IF, ELISA C7orf47 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C7orf47 fusion protein Ag18340
• Vollständiger Name: chromosome 7 open reading frame 47
• Berechnetes Molekulargewicht: 253 aa, 28 kDa
• GenBank-Zugangsnummer: BC026269
• Gene symbol: C7orf47
• Gene ID (NCBI): 221908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Elife; PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN.; Authors - Andrew Michael Sydor

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (04-24-2023)

Weak centriolar staining. While this antibody works well for Westernblotting, the expected centriolar staining in IF is rather weak. Yet, this antibody works under the following conditions: RPE1 cells stained for Hoechst (DNA marker, in green), PPP1r35 (centriolar marker, in magenta) and g-Tubulin (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:300 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

C7orf47 Antibody Immunofluorescence validation (1:50 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:24214-1-AP)