C6orf211/ARMT1 Polyklonaler Antikörper

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• KD/KO Validated

C6orf211/ARMT1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25124-1-AP

Synonyme

Acidic residue methyltransferase 1, ARMT1, C6orf211, Protein-glutamate O-methyltransferase

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 25124-1-AP

  WB result of C6orf211/ARMT1 antibody (25124-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C6orf211/ARMT1 transfected MCF-7 cells.

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  WB analysis of MCF-7 using 25124-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25124-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 25124-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25124-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25124-1-AP (C6orf211 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 25124-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25124-1-AP (C6orf211 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 25124-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25124-1-AP (C6orf211 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 25124-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25124-1-AP (C6orf211 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 25124-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25124-1-AP (C6orf211 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 25124-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 25124-1-AP (C6orf211 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A431-Zellen, HEK-293-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25124-1-AP bindet in WB, IHC, IF, ELISA C6orf211/ARMT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C6orf211/ARMT1 fusion protein Ag19090
• Vollständiger Name: chromosome 6 open reading frame 211
• Berechnetes Molekulargewicht: 441 aa, 51 kDa
• Beobachtetes Molekulargewicht: 50-51 kDa
• GenBank-Zugangsnummer: BC011348
• Gene symbol: C6orf211
• Gene ID (NCBI): 79624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.