cGAS Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

cGAS Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

63

Rezensionen (4)

Kat-Nr. : 26416-1-AP

Synonyme

C6orf150, cGAS, Cyclic GMP-AMP synthase, h-cGAS, MB21D1

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Galerie der Validierungsdaten

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  WB analysis using 26416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26416-1-AP (cGAS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26416-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cGAS antibody (26416-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 48 publications below
• IHC: See 9 publications below
• IF: See 22 publications below
• IP: See 1 publications below

Produktinformation

26416-1-AP bindet in WB, IP, IHC, IF, ELISA cGAS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: cGAS fusion protein Ag23455
• Vollständiger Name: chromosome 6 open reading frame 150
• Berechnetes Molekulargewicht: 496 aa, 54 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC113608
• Gene symbol: C6orf150
• Gene ID (NCBI): 115004
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

Publikationen

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
human	WB,IF	Cell; Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2 Regulatory Circuit.; Authors - Nathan Meade
	WB,IF	Adv Sci (Weinh); PICH Supports Embryonic Hematopoiesis by Suppressing a cGAS-STING-Mediated Interferon Response.; Authors - Xinwei Geng
human	WB	Nat Commun; ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling; Authors - Maximilian Hirschenberger
	WB	Mol Cell; CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity; Authors - Guiheng Zhang ; KO Validated
human	WB	J Clin Invest; Melatonin inhibits cytosolic mitochondrial DNA-induced neuroinflammatory signaling in accelerated aging and neurodegeneration.; Authors - Abhishek Jauhari

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sonia (Verified Customer) (09-12-2023)

Very clear staining of ruptured micronuclei in U2OS cells, fixed in 4% FA and permeabilised with PBS-Triton 0.5%. Primary antibody incubation was performed o/n at 4ºC in blocking buffer at 1:250.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: U2OS cells

FH

Shunbin (Verified Customer) (06-15-2023)

Produces a specific band and two other non-specific bands under the indicated conditions. The other cGAS Ab from Cell Signaling Technology produces one single band. (compare images attached

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

cGAS Antibody Western Blot validation (1:1000 dilution) in (Cat no:26416-1-AP)

FH

Zhiping (Verified Customer) (03-18-2022)

Good Ab for WB

• Applications: Western Blot

FH

Coralie (Verified Customer) (10-20-2021)

Cytosolic staining of cGAS (forms dots, after PFA fixation).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: HFF

cGAS Antibody Immunofluorescence validation (1/100 dilution) in HFF (Cat no:26416-1-AP)