C6 Polyklonaler Antikörper

C6 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 17239-1-AP

Synonyme

C6, complement component 6, Complement component C6

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Galerie der Validierungsdaten

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  WB analysis of human blood using 17239-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17239-1-AP (C6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 17239-1-AP

  IP Result of anti-C6 (IP:17239-1-AP, 4ug; Detection:17239-1-AP 1:800) with human plasma lysate 4000ug.

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  IHC staining of mouse liver using 17239-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17239-1-AP (C6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 17239-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17239-1-AP (C6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17239-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17239-1-AP (C6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 17239-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17239-1-AP (C6 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human blood
• Erfolgreiche IP: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

17239-1-AP bindet in WB, IP, IHC, IF, ELISA C6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C6 fusion protein Ag11083
• Vollständiger Name: complement component 6
• Berechnetes Molekulargewicht: 934 aa, 105 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC035723
• Gene symbol: C6
• Gene ID (NCBI): 729
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The final consequence of complement activation is formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. C6 is 1 of 5 late-acting proteins of complement that participate in assembly and function of the MAC. The deduced C6 protein contains 934 amino acids, including a 21-amino acid N-terminal signal sequence and 2 N-glycosylation sites. Mutations in the C6 gene are associated with complement component-6 deficiency.

Publikationen

Species	Application	Title
human	IF	PLoS One; Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury.; Authors - Shani Zilberman-Itskovich
human	WB	Oral Dis; Analysis of Salivary Exosomal Proteins in Young Adults with Severe Periodontitis.; Authors - Xiaoyi Huang
human	IHC	Ann Diagn Pathol; The histologic and molecular correlates of liver disease in fatal COVID-19 including with alcohol use disorder; Authors - Gerard J. Nuovo
rat	IF	Ren Fail; Effect of anti-C5 antibody on recuperation from ischemia/reperfusion-induced acute kidney injury.; Authors - Shani Zilberman-Itskovich
human	IHC	Ann Diagn Pathol; The amplification of CNS damage in Alzheimer's disease due to SARS-CoV2 infection; Authors - Gerard J. Nuovo
human	IF	Nat Commun; A ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells; Authors - Xue Li

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