C4BPB Polyklonaler Antikörper

C4BPB Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 15837-1-AP

Synonyme

C4b binding protein beta chain, C4BP, C4BPB

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 15837-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 15837-1-AP (C4BPB Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of mouse ovary using 15837-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15837-1-AP (C4BPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15837-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15837-1-AP (C4BPB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15837-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15837-1-AP (C4BPB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 15837-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15837-1-AP (C4BPB Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 15837-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15837-1-AP (C4BPB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, HeLa-Zellen, Maus-Eierstockgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

15837-1-AP bindet in WB, IHC, IF, ELISA C4BPB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C4BPB fusion protein Ag8587
• Vollständiger Name: complement component 4 binding protein, beta
• Berechnetes Molekulargewicht: 251 aa, 28 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC005378
• Gene symbol: C4BPB
• Gene ID (NCBI): 725
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C4b-binding protein (C4BP) is a large oligomeric glycoprotein, present in plasma at a concentration of approximately 200 mg/L (PMID: 24218259). It is the main inhibitor of the classical complement and lectin pathway. C4BP is composed of two polypeptides (alpha and beta chains), which have molecular weights of 70 and 45 kDa, respectively (PMID: 7561113). This polyclonal antibody is raised against the C4BP beta chain (C4BPB).

Publikationen

Species	Application	Title
human	WB	BMC Cancer; The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer.; Authors - Ping Zhao
human	WB	World J Surg Oncol; Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma; Authors - Wei Dong