C22orf25 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

C22orf25 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 27846-1-AP

Synonyme

C22orf25, TANGO2, Transport and Golgi organization protein 2 homolog

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 27846-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27846-1-AP (C22orf25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 27846-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27846-1-AP (C22orf25 antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human kidney using 27846-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27846-1-AP (C22orf25 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 27846-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27846-1-AP (C22orf25 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27846-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27846-1-AP (C22orf25 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27846-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27846-1-AP (C22orf25 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

27846-1-AP bindet in WB, IHC, IF, ELISA C22orf25 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C22orf25 fusion protein Ag27127
• Vollständiger Name: chromosome 22 open reading frame 25
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC041339
• Gene symbol: C22orf25
• Gene ID (NCBI): 128989
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Inherit Metab Dis; The phenotype associated with variants in TANGO2 may be explained by a dual role of the protein in ER-to-Golgi transport and at the mitochondria.; Authors - Miroslav P Milev ; KD Validated
human	WB	J Inherit Metab Dis; Clinical and biological characterization of 20 patients with TANGO2 deficiency indicates novel triggers of metabolic crises and no primary energetic defect.; Authors - Claire-Marine Bérat
human,mouse	WB	Nature; HRG-9 homologues regulate haem trafficking from haem-enriched compartments; Authors - Fengxiu Sun ; KO Validated
human	WB,IF	Dis Model Mech; Intrinsic and extrinsic regulation of rhabdomyolysis susceptibility by Tango2; Authors - Euri S Kim

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Silvia (Verified Customer) (09-30-2021)

Antibody works well ( band near 45-50 kDa)

• Applications: Western Blot