MGME1 Polyklonaler Antikörper

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• KD/KO Validated

MGME1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 23178-1-AP

Synonyme

C20orf72, DDK1, MGME1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 23178-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23178-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 23178-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23178-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 23178-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23178-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23178-1-AP (MGME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 23178-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23178-1-AP (MGME1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, Mauslebergewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

23178-1-AP bindet in WB, IP, IHC, IF, ELISA MGME1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MGME1 fusion protein Ag19644
• Vollständiger Name: chromosome 20 open reading frame 72
• Berechnetes Molekulargewicht: 344 aa, 39 kDa
• Beobachtetes Molekulargewicht: 36-39 kDa
• GenBank-Zugangsnummer: BC016869
• Gene symbol: C20orf72
• Gene ID (NCBI): 92667
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MGME1, also named as C20orf72, is a single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. It has preference for 5'-3' exonuclease activity. MGME1 cleaves single-stranded DNA (ssDNA) and several DNA or DNA-RNA chimeric oligonucleotides that showed free nucleic acid ends. It probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair. This antibody detects the 35-40 kDa protein.

Publikationen

Species	Application	Title
mouse	WB	Immunity; Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling.; Authors - Hongxu Xian ; KD Validated
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu ; KO Validated
mouse	IF,IP	mBio; Heat Shock Protein 60 Is Involved in Viral Replication Complex Formation and Facilitates Foot and Mouth Virus Replication by Stabilizing Viral Nonstructural Proteins 3A and 2C; Authors - Jianli Tang