COSMC Polyklonaler Antikörper

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• KD/KO Validated

COSMC Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 19254-1-AP

Synonyme

C1Gal T2, C1GALT1 chaperone 1, C1GALT1 specific chaperone 1, C1GALT1C1, C1GALT2, c38h2 l1, C38H2 like protein 1, Core 1 beta3 Gal T2, COSMC, HSPC067, MSTP143, UNQ273/PRO310

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 19254-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19254-1-AP

  WB result of COSMC antibody (19254-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COSMC transfected HepG2 cells.

• 

  WB analysis of HepG2 using 19254-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19254-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 19254-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 19254-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19254-1-AP (COSMC antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19254-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19254-1-AP (COSMC antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Caco-2-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

19254-1-AP bindet in WB, IHC, IF, ELISA COSMC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COSMC fusion protein Ag6899
• Vollständiger Name: C1GALT1-specific chaperone 1
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36-37 kDa
• GenBank-Zugangsnummer: BC050441
• Gene symbol: C1GALT1C1
• Gene ID (NCBI): 29071
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COSMC (C1GALT1C1) is a molecular chaperone required for expression of active T-synthase (CIGALT1) which catalyzes the synthesis of Tn antigen. Tn antigens are cancer-associated glycans and play an important role in tumor progression. Dysfunctional COSMC is able to convert a wild type protein into a tumor-specific antigen affecting tumor cell biology. COSMC overexpression had been observed in various cancers. COSMC mutations have been observed in an autoimmune disease Tn syndrome.

Publikationen

Species	Application	Title
human,mouse	WB	Aging Cell; Elevated miR-124-3p in the aging colon disrupts mucus barrier and increases susceptibility to colitis by targeting T-synthase.; Authors - Li Huang
human	WB	Aging (Albany NY); Cosmc transfection decreases malignant behavior of Tn + cells and enhances sensitivity to apoptosis when induced by Apo2L/TRAIL via alteration of O-glycan structure; Authors - Ruisong Ding
human	WB	Mol Cancer; COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer.; Authors - Bianca T Hofmann
human	WB,IF	Biochim Biophys Acta Mol Cell Res; SLC35A2 deficiency reduces protein levels of core 1 β-1,3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localization; Authors - Wojciech Wiertelak
human	IHC	Respir Physiol Neurobiol; The Cosmc-mediated effects of neutrophil elastase on T antigen expression in BEAS-2B cells.; Authors - Lin Luo
mouse	WB	Foods; Si-Wu Water Extracts Protect against Colonic Mucus Barrier Damage by Regulating Muc2 Mucin Expression in Mice Fed a High-Fat Diet; Authors - Zheng Ruan