C16orf84 Polyklonaler Antikörper

C16orf84 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23337-1-AP

Synonyme

C16orf84, CTU2, NCS2, UPF0432

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 23337-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 23337-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 23337-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 23337-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 23337-1-AP (C16orf84 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 23337-1-AP

  IP Result of anti-C16orf84 (IP:23337-1-AP, 4ug; Detection:23337-1-AP 1:500) with HepG2 cells lysate 3600ug.

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  IHC staining of human kidney using 23337-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 23337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 23337-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 23337-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23337-1-AP (C16orf84 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HEK-293 using 23337-1-AP

  Immunofluorescent analysis of HEK-293 cells using 23337-1-AP (C16orf84 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mauslebergewebe, Mausmilzgewebe, Raji-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23337-1-AP bindet in WB, IP, IHC, IF,ELISA C16orf84 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C16orf84 fusion protein Ag18428
• Vollständiger Name: chromosome 16 open reading frame 84
• Berechnetes Molekulargewicht: 515 aa, 56 kDa
• Beobachtetes Molekulargewicht: 65-68 kDa
• GenBank-Zugangsnummer: BC125269
• Gene symbol: C16orf84
• Gene ID (NCBI): 348180
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytoplasmic tRNA 2-thiolation protein 2 (CUT2), also named as C16orf84 or NCS2, is a 515 amino acid protein, which belongs to the CUT2 family. C16orf84 localizes in the cytoplasm and Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). C16orf84 may act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thio-carboxylated URM1 onto the uridine of tRNAs at wobble position.