Bcl-XL Monoklonaler Antikörper

Bcl-XL Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Hund

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

4C12A6

Kat-Nr. : CL594-66020

Synonyme

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL, DKFZp781P2092

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Galerie der Validierungsdaten

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  IF Staining of HeLa using CL594-66020

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66020 (Bcl-XL antibody) at dilution of 1:100.

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  IF Staining of MDCK using CL594-66020

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using CoraLite®594-conjugated Bcl-XL antibody (CL594-66020, Clone: 4C12A6 ) at dilution of 1:200.

• 

  FC experiment of HeLa using CL594-66020

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Bcl-XL (CL594-66020, Clone:4C12A6) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen, MDCK-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66020 bindet in IF, FC (Intra) Bcl-XL und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Bcl-XL fusion protein Ag18037
• Vollständiger Name: BCL2-like 1
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC019307
• Gene symbol: BCL2L1
• Gene ID (NCBI): 598
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011