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Bcl-XL Monoklonaler Antikörper

Bcl-XL Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

4C12A6

Kat-Nr. : CL555-66020

Synonyme

Apoptosis regulator Bcl X, BCL2L1, DKFZp781P2092

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated Bcl-XL Monoclonal antibody (CL555-66020)

    IF Staining of HeLa using CL555-66020

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 Bcl-XL antibody (CL555-66020, Clone: 4C12A6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite®555-conjugated Bcl-XL Monoclonal antibody (CL555-66020)

    FC experiment of Jurkat using CL555-66020

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite®555 Anti-Human Bcl-XL (CL555-66020, Clone:4C12A6) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCJurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL555-66020 bindet in IF, FC (Intra) Bcl-XL und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Bcl-XL fusion protein Ag18037
Vollständiger Name BCL2-like 1
Berechnetes Molekulargewicht 26 kDa
Beobachtetes Molekulargewicht 30 kDa
GenBank-ZugangsnummerBC019307
Gene symbol BCL2L1
Gene ID (NCBI) 598
Konjugation CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

Protokolle

Produktspezifische Protokolle
IF protocol for CL555 Bcl-XL antibody CL555-66020Protokoll herunterladen
FC protocol for CL555 Bcl-XL antibody CL555-66020Protokoll herunterladen
Standard-Protokolle
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