• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Bcl-XL Monoklonaler Antikörper

Bcl-XL Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Hund

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4C12A6

Kat-Nr. : CL488-66020

Synonyme

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL, DKFZp781P2092

Galerie der Validierungsdaten

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Bcl-XL Monoclonal an (CL488-66020)

    IF Staining of HeLa using CL488-66020

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL488-66020 (Bcl-XL antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CoraLite® Plus 488-conjugated Bcl-XL Monoclonal an (CL488-66020)

    IF Staining of MDCK using CL488-66020

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using CoraLite® Plus 488-conjugated Bcl-XL antibody (CL488-66020, Clone: 4C12A6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated Bcl-XL Monoclonal an (CL488-66020)

    FC experiment of Jurkat using CL488-66020

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Bcl-XL (CL488-66020, Clone:4C12A6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen, MDCK-Zellen
Erfolgreiche Detektion in FCJurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66020 bindet in IF, FC (Intra) Bcl-XL und zeigt Reaktivität mit human, Hund

Getestete Reaktivität human, Hund
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Bcl-XL fusion protein Ag18037
Vollständiger Name BCL2-like 1
Berechnetes Molekulargewicht 26 kDa
GenBank-ZugangsnummerBC019307
Gene symbol BCL2L1
Gene ID (NCBI) 598
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 Bcl-XL antibody CL488-66020Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen