BRIP1(N-terminal) Polyklonaler Antikörper

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• KD/KO Validated

BRIP1(N-terminal) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 24436-1-AP

Synonyme

BACH1, BRIP1, BRIP1(N-terminal), FANCJ, OF

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Galerie der Validierungsdaten

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  WB analysis using 24436-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24436-1-AP (BRIP1(N-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24436-1-AP

  WB result of BRIP1(N-terminal) antibody (24436-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BRIP1(N-terminal) transfected HeLa cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

24436-1-AP bindet in WB, ELISA BRIP1(N-terminal) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BRIP1(N-terminal) fusion protein Ag19907
• Vollständiger Name: BRCA1 interacting protein C-terminal helicase 1
• Berechnetes Molekulargewicht: 1249 aa, 141 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC101472
• Gene symbol: BRIP1
• Gene ID (NCBI): 83990
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRIP1 also named as BACH1, FANCJ or ATP dependent RNA helicase BRIP1 is a 1249 amino acid protein, which contains one helicase ATP-binding domain and belongs to the DEAD box heilicase family, DEAH subfamily. BRIP1 contains nuclear localization signal and is ubiquitously expressed with highest levels in testis, an expression pattern similar to that of BRCA1. BRIP1 is required to resolve the secondary structures of guanine-rich DNA that occasionally form during lagging-strand DNA synthesis.

Publikationen

Species	Application	Title
human	WB	Mol Cell; A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.; Authors - Jiao Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Adrianna (Verified Customer) (10-31-2022)

This antibody is not as good as some of the other Proteintech antibodies (it has more background) and it looks like I am detecting two bands, however I can clearly see the correct band at around 140 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7