BRD7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BRD7 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Kat-Nr. : 51009-2-AP

Synonyme

75 kDa bromodomain protein, BP75, BRD7, bromodomain containing 7, CELTIX1, NAG4, Protein CELTIX 1

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 51009-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51009-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51009-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 51009-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 51009-2-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 51009-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 51009-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 51009-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 51009-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51009-2-AP (BRD7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 51009-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51009-2-AP (BRD7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 51009-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51009-2-AP (BRD7 Antibody) at dilution of 1:50 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, Jurkat-Zellen, L02-Zellen, Maus-Cerebellum-Gewebe, Mauslungengewebe, MDA-MB-231-Zellen, PC-3-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 23 publications below
• IHC: See 9 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

51009-2-AP bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA BRD7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BRD7 fusion protein Ag3911
• Vollständiger Name: bromodomain containing 7
• Berechnetes Molekulargewicht: 651 aa, 74 kDa
• Beobachtetes Molekulargewicht: 70-74 kDa
• GenBank-Zugangsnummer: BC050728
• Gene symbol: BRD7
• Gene ID (NCBI): 29117
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.

Publikationen

Species	Application	Title
human	WB	Cancer Discov; A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction.; Authors - Jinxiu Li
mouse	IF	Nat Commun; SS18 regulates pluripotent-somatic transition through phase separation.; Authors - Junqi Kuang
human	WB	Cell Rep; FAK-mediated phosphorylation at Y464 regulates p85β nuclear translocation to promote tumorigenesis of ccRCC by repressing RB1 expression; Authors - Yanhua Zhang
human	WB,CoIP	Cancer Res; BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription.; Authors - Harte Mary T MT ; KD Validated
mouse	WB	Cell Mol Immunol; BRD7 plays an anti-inflammatory role during early acute inflammation by inhibiting activation of the NF-кB signaling pathway.; Authors - Ran Zhao ; KO Validated
human	WB,IHC	Oncotarget; Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma.; Authors - Chang-Long Chen