BOULE Polyklonaler Antikörper

BOULE Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-13720

Synonyme

bol, boule like (Drosophila), BOLL, BOULE, Protein boule like

---

Galerie der Validierungsdaten

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:400, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite® Plus 647 TNP1 antibody (CL647-17178, Magenta).

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:400, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite® Plus 647 TNP1 antibody (CL647-17178, Magenta).

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (cold ethanol) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:100, CoraLite®555 TNP1 antibody (CL555-17178, orange), STAR antibody (67130-1-Ig, Clone: 1G6E6, Magenta).

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200, CoraLite®594 PRM1 antibody (CL594-15697, red).

• 

  IF Staining of mouse testis using CL488-13720

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:200, CoraLite®594 PRM1 antibody (CL594-15697, red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maushodengewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-13720 bindet in IF BOULE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BOULE fusion protein Ag4662
• Vollständiger Name: bol, boule-like (Drosophila)
• Berechnetes Molekulargewicht: 283 aa, 31 kDa
• GenBank-Zugangsnummer: BC033674
• Gene symbol: BOLL
• Gene ID (NCBI): 66037
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).