BOP1 Polyklonaler Antikörper

BOP1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 28366-1-AP

Synonyme

block of proliferation 1, BOP1, KIAA0124

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Galerie der Validierungsdaten

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  WB analysis using 28366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28366-1-AP (BOP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 28366-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 28366-1-AP (BOP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 28366-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 28366-1-AP (BOP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 28366-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28366-1-AP (BOP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28366-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28366-1-AP (BOP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28366-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28366-1-AP (BOP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28366-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28366-1-AP (BOP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 28366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using 28366-1-AP (BOP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 28366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 28366-1-AP (BOP1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HeLa-Zellen, NCI-H1299-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

28366-1-AP bindet in WB, IHC, IF BOP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BOP1 fusion protein Ag26669
• Vollständiger Name: block of proliferation 1
• Berechnetes Molekulargewicht: 84 kDa
• Beobachtetes Molekulargewicht: 117 kDa
• GenBank-Zugangsnummer: BC013787
• Gene symbol: BOP1
• Gene ID (NCBI): 23246
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Block of Proliferation 1(BOP1) protein was first isolated from a mouse, and the truncation protein may led to cell cycle arrest. Bop1 plays a role in processing of the 28S and 5.8S rRNAs (ribosomal RNAs) to regulate ribosome biogenesis. And Bop1 also act as a component of Pes1/Bop1/WDR21 (PeBoW) complex for mature 60S ribosome, and consequently acts during cell division at G1 checkpoints (PMID:18670790; PMID:26940494). What's more, abnormal expression of BOP1 was associated with liver or colorectal cancers(PMID:16804918). The molecular weight of the protein we detected was slightly higher, about 117 kd, which is also seen in the article(PMID: 30100995).

Publikationen

Species	Application	Title
human	WB	Int J Biol Sci; Discovery of a novel HDACi structure that inhibits the proliferation of ovarian cancer cells in vivo and in vitro.; Authors - Miao Bai
human	WB	Front Oncol; Suppression of Ribosome Biogenesis by Targeting WD Repeat Domain 12 (WDR12) Inhibits Glioma Stem-Like Cell Growth.; Authors - Lanjuan Mi