BNIP3L Monoklonaler Antikörper

BNIP3L Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F6A3

Kat-Nr. : 68118-1-Ig

Synonyme

BNIP3a, BNIP3H, BNIP3L, NIP3 like protein X, NIP3L, NIX

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Galerie der Validierungsdaten

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  WB analysis using 68118-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68118-1-Ig (BNIP3L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  IHC staining of human prostate cancer using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68118-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68118-1-Ig (BNIP3L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 68118-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BNIP3L antibody (68118-1-Ig, Clone: 1F6A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, MOLT-4-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Rattennierengewebe, humanes Prostatakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68118-1-Ig bindet in WB, IHC, IF, ELISA BNIP3L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BNIP3L fusion protein Ag31547
• Vollständiger Name: BCL2/adenovirus E1B 19kDa interacting protein 3-like
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 35 kD
• GenBank-Zugangsnummer: BC001559
• Gene symbol: BNIP3L
• Gene ID (NCBI): 665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.