BNIP3 Monoklonaler Antikörper

BNIP3 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

CloneNo.

2H7D8

Kat-Nr. : 68091-1-Ig

Synonyme

BNIP3, NIP3

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 68091-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68091-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 68091-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 68091-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using BNIP3 antibody (68091-1-Ig, Clone: 2H7D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF: mit Cobaltchlorid behandelte HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 2 publications below

Produktinformation

68091-1-Ig bindet in WB, IHC, IF, ELISA BNIP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BNIP3 fusion protein Ag27935
• Vollständiger Name: BCL2/adenovirus E1B 19kDa interacting protein 3
• Berechnetes Molekulargewicht: 194 aa, 22 kDa
• Beobachtetes Molekulargewicht: 25-30,50-60 kDa
• GenBank-Zugangsnummer: BC009342
• Gene symbol: BNIP3
• Gene ID (NCBI): 664
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.

Publikationen

Species	Application	Title
mouse	WB	Cell Biosci; Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy; Authors - Mei-Fei Wu
human	WB	Front Cell Dev Biol; Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma; Authors - Wenshuo Zhang
mouse	WB,IHC	Cell Commun Signal; Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1; Authors - Xudong Xie
rat	IHC	Biol Trace Elem Res; The Role of BNIP3 and Blocked Autophagy Flux in Arsenic-Induced Oxidative Stress-Induced Liver Injury in Rats; Authors - Haiyan Zhi
	WB	Equine Vet J; Muscle fibre transition and transcriptional changes of horse skeletal muscles during traditional Mongolian endurance training; Authors - Tugeqin Bou
human	WB	ACS Appl Mater Interfaces; Arsenic Prodrug-Mediated Tumor Microenvironment Modulation Platform for Synergetic Glioblastoma Therapy; Authors - Jiliang Yan