BMF Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BMF Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 18298-1-AP

Synonyme

BMF

---

Galerie der Validierungsdaten

• 

  WB analysis of human placenta using 18298-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18298-1-AP (BMF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18298-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18298-1-AP (BMF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

18298-1-AP bindet in WB, ELISA BMF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Bcl2 modifying factor
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC069505
• Gene symbol: BMF
• Gene ID (NCBI): 90427
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BMF belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities such as central regulators of apoptotic cell death. BMF contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. BMF is widely expressed in tissues such as pancreas, liver and kidney, and in hematopoietic tissues.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Characterization of an alternative BAK-binding site for BH3 peptides.; Authors - Kaiqin Ye
human	WB	Int J Biol Sci; RBMS2 Chemosensitizes Breast Cancer Cells to Doxorubicin by Regulating BMF Expression.; Authors - Feng Xu ; KD Validated
human	WB	PLoS One; Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli.; Authors - Yu Guohua G
mouse	WB	Aging Dis; BMF-AS1/BMF Promotes Diabetic Vascular Calcification and Aging both In Vitro and In Vivo; Authors - Xiao Lin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Chi (Verified Customer) (09-28-2019)

This antibody generally works well to detect BMF in western blot at a relatively high concentration.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse embryonic fibroblasts

BMF Antibody Western Blot, validation (1:500 dilution) in mouse embryonic fibroblasts (Cat no:18298-1-AP)