BLOC1S1-Specific Polyklonaler Antikörper

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• KD/KO Validated

BLOC1S1-Specific Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 19687-1-AP

Synonyme

BLOC 1 subunit 1, BLOC1S1, BLOC1S1-Specific, BLOS1, GCN5 like protein 1, GCN5L1, MICoA, Protein RT14, RT14

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Galerie der Validierungsdaten

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  WB analysis of human brain using 19687-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19687-1-AP (BLOC1S1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 19687-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19687-1-AP (BLOC1S1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19687-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19687-1-AP (BLOC1S1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19687-1-AP

  IP Result of anti-BLOC1S1-Specific (IP:19687-1-AP, 3ug; Detection:19687-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human small intestine using 19687-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine slide using 19687-1-AP (BLOC1S1-Specific Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

19687-1-AP bindet in WB, IP, IHC, IF, ELISA BLOC1S1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: biogenesis of lysosomal organelles complex-1, subunit 1
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 14-17 kDa
• GenBank-Zugangsnummer: NM_001487
• Gene symbol: BLOC1S1
• Gene ID (NCBI): 2647
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BLOC1S1, also named as BLOS1, GCN5L1 and RT14, belongs to the BLOC1S1 family. BLOC1S1 may be involved in the biogenesis of specialized organelles of the endosomal-lysosomal system. The antibody is specific to NLOC1S1.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Decoding non-canonical mRNA decay by the endoplasmic-reticulum stress sensor IRE1α; Authors - Adrien Le Thomas
mouse	WB	Circ Res; NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.; Authors - Dan Tong
human	WB	EMBO J; Snapin promotes HIV-1 transmission from dendritic cells by dampening TLR8 signaling.; Authors - Elham Khatamzas ; KD Validated
human	WB	Autophagy; HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons.; Authors - Maryline Santerre
human	WB	Oxid Med Cell Longev; General Control of Amino Acid Synthesis 5-Like 1-Mediated Acetylation of Manganese Superoxide Dismutase Regulates Oxidative Stress in Diabetic Kidney Disease.; Authors - Tingting Lv
	WB,IHC,IF,IP	FEBS J; A guide to assessing endoplasmic reticulum homeostasis and stress in mammalian systems.; Authors - Daria Sicari